IEC-18細(xì)胞

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

ATCC Number： CRL-1589?

細(xì)胞類型： 上皮細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

器官來源： 其他

Designations: IEC-18

Depositors: A Quaroni

IEC-18細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: ileum

Cell Type: epithelial

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

IEC-18細(xì)胞Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: IEC-18細(xì)胞A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 22491: Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453

26096: Quaroni A, Isselbacher KJ. Cytotoxic effects and metabolism of benzo[a]pyrene and 7,12- dimethylbenz[a]anthracene in duodenal and ileal epithelial cell cultures. IEC-18細(xì)胞J. Natl. Cancer Inst. 67: 1353-1362, 1981. PubMed: 6273638