TF-1細(xì)胞

器官來源： 骨髓

ATCC Number： CRL-2003?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 白血病

細(xì)胞形態(tài)： **樣

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來源： 人

生長狀態(tài)： 懸浮生長

年限： 35 years

數(shù)量： 大量

Designations: TF-1

Depositors: T Kitamura

TF-1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: bone marrow

Disease: erythroleukemia

Cell Type: erythroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche Transfection Reagents)

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 13,14

D13S317: 8,9

D16S539: 9,12

TF-1細(xì)胞D5S818: 13

D7S820: 12

THO1: 7,9

TPOX: 8

vWA: 15,17

Age: 35 years

Gender: male

Ethnicity: Japanese

Comments: The TF-1 cell line was established by T. Kitamura, et al. in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia.The cells are completely dependent on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. The cells DO NOT RESPOND to interleukin 5 (IL-5).TF-1 cells respond to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9),Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF).TF-1 cells do not express glycophorin A or carbonyl anhydrase I.The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage.TF-1細(xì)胞 Hemin and delta-aminolevulinic acid induce hemoglobin synthesis, and TPA induces dramatic differentiation of the TF-1 cells into macrophage-like cells. The TF-1 cell line is unique because of its responsiveness to multiple cytokines and provides a good system for investigating the proliferation and differentiation of myeloid progenitor cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2 ng/ml recombinant human GM-CSF; fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 7 X 10(5) cells/ml.

Interval: Maintain cultures between 3 X 10(4) and 5 X 10(5) viable cells/ml

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: TF-1細(xì)胞liquid nitrogen vapor phase

Doubling Time: 22 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derivative:ATCC CRL-2451

derivative:ATCC CRL-2512

References: 22415: Kitamura T, et al. IL-1 up-regulates the expression of cytokine receptors on a factor- dependent human hemopoietic cell line, TF-1. Int. Immunol. 3: 571-577, 1991. PubMed: 1832294

22619: Kitamura T, et al. Identification and analysis of human erythropoietin receptors on a factor-dependent cell line, TF-1. Blood 73: 375-380, 1989. PubMed: 2537111

23007: Kitamura T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334, 1989. PubMed: 2663885

23415: Borellini F, Glazer RI. Induction of Sp1-p53 DNA-binding heterocomplexes during granulocyte/macrophage colony-stimulating factor-dependent proliferation in human erythroleukemia cell line TF-1. J. Biol. Chem. 268: 7923-7928, 1993. PubMed: 8463313

30089: Schulte T, et al. Molecular characterization and functional analysis of murine interleukin 4 receptor allotypes. J. Exp. Med. 186: 1419-1429, 1997. PubMed: 9348299

30146: Mueller CG, et al. TF-1細(xì)胞Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells. J. Exp. Med. 186: 655-663, 1997. PubMed: 9271581

30515: Limon A, et al. High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells. Blood 90: 3316-3321, 1997. PubMed: 9345013

49300: Hu X, et al. Prolonged activation of the mitogen-activated protein kinase pathway is required for macrophage-like differentiation of a human myeloid leukemic cell line. Cell Growth Differ. 11: 191-200, 2000. PubMed: 10775036

92234: USP Monographs: Sargramostim. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011