HS-27A細(xì)胞

ATCC Number： CRL-2496?

細(xì)胞形態(tài)： 上皮樣

細(xì)胞類型： 其他細(xì)胞類型

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 人

數(shù)量： 大量

器官來源： 骨髓

生長狀態(tài)： 貼壁生長

組織來源： stroma

年限： 30 years

Designations: HS-27A

Depositors: B Torok-Storb

HS-27A細(xì)胞Biosafety Level: 2 [Cells contain human papilloma viral sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: epithelial

Source: Organ: bone marrow

Tissue: stroma

Cell Type: human papillomavirus 16 (HPV-16) E6/E7 transformed

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: These cells are distributed for research purposes only. The line is available with the following restrictions:1. This cell line was deposited at the ATCC by Dr. B. Torok-Storb.The line is provided for research purposes only and is available under the condition that the material will not be used for commercial purposes or be distributed to third parties. HS-27A細(xì)胞The cells are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.2. Any proposed commercial use of the these cells, or their products must first be negotiated with the Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, C2M-027M, Seattle, WA 98109. Telephone (206) 667-6304, FAX (206-667-4732).

Antigen Expression: vascular cell adhesion molecule 1 (CD106, VCAM-1)

Age: 30 years

Gender: male

Ethnicity: Caucasian

Comments: Long term bone marrow cells were transformed with the amphotropic retrovirus vector LXSN16E6E7 in the presence of polybrene.

Twenty-seven immortalized clones designed HS-1 to HS-27 were isolated.

HS-27A is a subclone of HS-27.

One of these cell lines HS-27A (CRL-2496) has been deposited in the ATCC 's general collection. HS-27A細(xì)胞One cell line (HS-5) has been deposited in the Patent Depository (CRL-11882).

HS-27A forms large flattened polygonal shaped cells that exemplify "blanket" cells and maintain numerous intercellular contacts with neighboring cells. [44213]

Unlike HS-5, HS-27A secretes low levels of growth factors and does not support proliferation of isolated progenitor cells in cocultures.

HS-27A expresses relatively high levels of vascular cell adhesion molecule 1 (VCAM-1). [40180]

HS-27A supports the formation of "cobblestone" areas by isolated cells positive for CD34 but low for CD38. [40180]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended

Medium Renewal: HS-27A細(xì)胞Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 40180: Roecklein BA, Torok-Storb B. Functionally distinct human marrow stromal cell lines immortalized by transduction with the human papilloma virus E6/E7 genes. Blood 85: 997-1005, 1995. PubMed: 7849321

44213: Torok-Storb B, et al. Human marrow stromal cell lines which sustain hematopoieses. US Patent 5,879,940 dated Mar 9 1999