MH-S細胞
數(shù)量： 大量
ATCC Number： CRL-2019?
生長狀態(tài)： 混合型生長
器官來源： 肺
年限： 7 weeks
細胞形態(tài)： 單核細胞/巨噬細胞
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 小鼠
品系： BALB/c
運輸方式： 凍存運輸
Designations: MH-S
Depositors: I Mbawuike, HB Herscowitz
Biosafety Level: 2 [Cells may contain SV40 virus ]
Shipped: MH-S細胞frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Mus musculus
Morphology: macrophage
Source: Organ: lung
Strain: BALB/c
Cell Type: macrophage (alveolar);
Cellular Products: interleukin 1 (IL-1)
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Receptors: Fc
Antigen Expression: CD11b (Mac-1); Class II antigens (I-A); T antigen
Age: 7 weeks
Gender: male
Comments: MH-S細胞The MH-S cell line was derived by SV40 transformation of an adherent cell enriched population of mouse alveolar macrophages.
The cells retain many of the properties of alveolar macrophages including typical macrophage morphology.
They are adherent, phagocytic, esterase positive and peroxidase negative.
Lipopolysaccharide (LPS) treatment stimulates IL-1 production.
The cells are capable of suppressing the in vitro plaque forming cell (PFC) response in a cell dose dependent manner.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by transferring floating cells to a centrifuge tube Rinse adherent cells with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. MH-S細胞Add to floating cells collected above and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22783: Mbawuike IN, Hercowitz HB. MH-S, a murine alveolar macrophage cell line: morphological, cytochemical, and functional characteristics. J. Leukocyte Biol. 46: 119-127, 1989.MH-S細胞 PubMed: 2787372
