NRK-52E細胞
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
細胞形態(tài)： 上皮樣
是否是腫瘤細胞： 0
物種來源： 大鼠
ATCC Number： CRL-1571?
數(shù)量： 大量
器官來源： 腎臟
相關(guān)**： 正常
Designations: NRK-52E
Depositors: JE DeLarco
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
NRK-52E細胞Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. NRK-52E細胞Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Receptors: epidermal growth factor (EGF); multiplication stimulating activity (MSA)
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.02% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. NRK-52E細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh culture medium, aspirate and dispense into new culture vessels.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2030
References: 22713: de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation.NRK-52E細胞 J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450
23197: Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742
