Fcwf-4細(xì)胞

年限： fetus

器官來(lái)源： 胚胎

細(xì)胞類型： 其他細(xì)胞類型

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 貓

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-2787?

細(xì)胞形態(tài)： 其他

Designations: Fcwf-4 [Fcwf]

Depositors: NC Pedersen

Fcwf-4細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Felis catus

Morphology: spindle to stellate

Source: Organ: fetus, whole

Cell Type: macrophage

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Isolation: Isolation date: 1979

Virus Susceptibility: Feline coronavirus

Age: fetus

Comments: This continuous feline cell line was developed in 1979. It is used to propagate tissue culture adapted Feline coronavirus (Feline infectious peritonitis virus, FIPV). The cells possess some characteristics of macrophages (nonspecific esterase, phagocytic activity, Fc receptors).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Fcwf-4細(xì)胞Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

An inoculum of 1 X 10(4) to 1 X 10(5) viable cells/cm2 is recommended. Do not exceed 1 X 10(6) cells/cm2..

Incubate cultures at 37?C.

Interval: Maintain cultures at a cell concentration between 2 X 10(4) and 1 X 10(5) cells/cm2.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Two to three times weekly

Preservation: Freeze medium: Fcwf-4細(xì)胞Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 31 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 71888: Jacobse-Geels HE, Horzinek MC. Expression of feline infectious peritonitis coronavirus antigens on the surface of feline macrophage-like cells. J. Gen. Virol. 64: 1859-2866, 1983. PubMed: 6886678

Fcwf-4細(xì)胞71889: Pedersen NC, et al. Infection studies in kittens, using feline infectious peritonitis virus propagated in cell culture. Am. J. Vet. Res. 42: 363-367, 1981. PubMed: 6267959