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產(chǎn)品資料

KG-1細(xì)胞

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產(chǎn)品名稱: KG-1細(xì)胞
產(chǎn)品型號: KG-1
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

KG-1細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。KG-1細(xì)胞何時須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


KG-1細(xì)胞  的詳細(xì)介紹

KG-1細(xì)胞

器官來源: 骨髓

生長狀態(tài): 懸浮生長

年限: 59 years

ATCC Number: CRL-8031?

相關(guān)**: 其他**

運輸方式: 凍存運輸

細(xì)胞形態(tài): **樣

數(shù)量: 大量

是否是腫瘤細(xì)胞: 0

物種來源: 人

Designations: KG-1

KG-1細(xì)胞Depositors: Genentech, Inc.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Organ: bone marrow

Disease: acute myelogenous leukemia

Cellular Products: human leukocyte interferon

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 7

D13S317: 11,12

D16S539: 10,11

D5S818: 13

D7S820: 8,10

THO1: 7,8

TPOX: 7,9

vWA: 14,19

Age: 59 years

Gender: male

Ethnicity: Caucasian

KG-1細(xì)胞Comments: Responsive to colony stimulating activity (CSA).

Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.

See also ATCC CCL-246.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. KG-1細(xì)胞Start cultures at 3 X 10(5) viable cells/ml.

Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1056: Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

4001: Goeddel DV. KG-1細(xì)胞Hybrid human leukocyte interferons. US Patent 4,678,751 dated Jul 7 1987

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