JC細(xì)胞

年限： 1.5 years

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 乳腺

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

品系： BALB/cRos

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-2116?

相關(guān)**： 腺癌

Designations: JC

Depositors: TM Chu

JC細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: mammary gland

Strain: BALB/cRos

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. JC細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Cytogenetic Analysis: modal number = 40; range = 26 to 100

Age: 1.5 years

Gender: female

JC細(xì)胞Comments: JC is an epithelial-like cell line established in 1983 from a spontaneous primary mammary adenocarcinoma along the milk line.

The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months.

Electron microscopic examination detected the presence of cellular tonofilaments and desmosomes.

No type B or C virus particles were detected, although intracisternal A particles were observed occasionally.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Twice per week

Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution and remove trypsin. Add fresh trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach. JC細(xì)胞Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Doubling Time: 12 to 16 hrs

References: 22452: Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534

22679: Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384