32D Clone 3細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： CRL-11346?

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

器官來源： 骨髓

生長狀態(tài)： 混合型生長

細(xì)胞形態(tài)： **樣

Designations: 32D Clone 3

Depositors: Amgen, Inc.

Biosafety Level: 1

Shipped: frozen

32D Clone 3細(xì)胞Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: bone marrow

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Applications: transfection host

Comments: This line is IL-3 dependent.

The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.

The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.

Propagation: 32D Clone 3細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with:10% heat-inactivated fetal bovine serum10% mouse Interleukin-3 culture supplement (available from Becton Dickinson Cat. No. 354040, formerly Collaborative Research Cat. No. 40040)

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. 32D Clone 3細(xì)胞Scrape off the attached cells and transfer along with the floating cells into new flasks.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

References: 49723: Ahmed N, Berridge MV. Regulation of glucose transport by interleukin-3 in growth factor-dependent and oncogene-transformed bone marrow-derived cell lines. Leuk. Res. 21: 609-618, 1997. PubMed: 9301681

50750: Ahmed N, Berridge MV. 32D Clone 3細(xì)胞Distinct regulation of glucose transport by interleukin-3 and oncogenes in a murine bone marrow-derived cell line. Biochem. Pharmacol. 57: 387-396, 1999. PubMed: 9933027