NRK-49F細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 成纖維樣

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 大鼠

ATCC Number： CRL-1570?

相關(guān)**： 正常

器官來(lái)源： 腎臟

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

Designations: NRK-49F

Depositors: JE DeLarco

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

NRK-49F細(xì)胞Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: fibroblast

Source: Organ: kidney

Disease: normal

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Applications: transfection host

Receptors: epidermal growth factor (EGF); multiplication stimulating activity (MSA)

Comments: NRK-49F cells are contact inhibited and very sensitive to transformation by chemicals and viruses.

Cultures should be subcultured before they become confluent.

If allowed to become confluent, the cells will transform.

Propagation: NRK-49F細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 22713: de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation. NRK-49F細(xì)胞J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450

23197: Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742

32442: Sachsenmeier KF, et al. Transforming growth factor-beta1 inhibits nucleosomal fragmentation in human keratinocytes following loss of adhesion. J. Biol. Chem. 271: 5-8, 1996. PubMed: 8550610

32705: Schultz-Cherry S, Hinshaw VS. Influenza virus neuraminidase activates latent transforming growth factor beta. J. Virol. 70: 8624-8629, 1996. PubMed: 8970987

33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

33159: Centrella M, et al. NRK-49F細(xì)胞Rapid flux in transforming growth factor-beta receptors on bone cells. J. Biol. Chem. 271: 18616-18622, 1996. PubMed: 8702513