PLHC-1細(xì)胞

ATCC Number： CRL-2406?

數(shù)量： 大量

相關(guān)**： 肝癌

器官來(lái)源： 肝

細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 其他

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

Designations: PLHC-1

PLHC-1細(xì)胞Depositors: LE Hightower

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Poeciliopsis lucida

Morphology: epithelial

Source: Organ: liver

Disease: hepatocellular carcinoma

Cell Type: hepatocyte;

Cellular Products: cytochrome P450 (CYP)

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Receptors: aryl hydrocarbon (Ah)

Gender: female

Comments: PLHC-1細(xì)胞The PLHC-1 (Poeciliopsis lucida hepatocellular carcinoma) cell line was derived from an ***** female Poeciliopsis lucida, a topminnow from the Sonoran Desert. [38901]

A transplantable tumor was induced by multiple doses of 7, 12-dimethylbenz(a)anthracene (DMBA) treatment of the fish in 1982 by Mary E. Schultz. [38891]

The tumor was adapted to cell culture in 1985 by Lawrence E. Hightower.

These cells maintain a number of differentiated cell functions of hepatocytes. The cells possess inducible and stable cytochrome P450 (CYF) activity. This activity is inducible by 3,3',4,4'-tetrachlorobiphenyl (TCB). [38907]

Cytochrome P450 activity is also inducible by Aroclor 1254 (a chemical inducer of cytochrome P450-dependent monooxygenase activity), and reduced by EPN (an inhibitor of P450 activity). [38898]

PHLC-1 cells can be used in an in vitro system to screen environmentally relevant stressors such as heavy metals using a combined stress protein and cytotoxicity assay. Increases in heat shock protein hsp70 can be detected in this system. [38901]

Retention of hepatocyte properties has made this cell line ideal for in vitro toxicology assays; it has been well characterized by environmental toxicologists.

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 95%; fetal bovine serum, 5%

Temperature: 30.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: PLHC-1細(xì)胞A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Briefly rinse the cell layer with 0.05% Trypsin-0.53 mM EDTA solution. Add 2.0 to 3.0 ml additional Trypsin-EDTA solution to flask and allow flask to set at room temperature for 3 to 5 minutes until cells detach.

Tap flask and cells will slide off bottom of the flask.

Note: Cells come off in small clumps and are difficult to break up into single cells without greatly reducing cell viability.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

References: 38889: Celander M, et al. Cytochromes P450 (CYP) in the Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1): dose- and time-dependent glucocorticoid potentiation of CYP1A induction without induction of CYP3A. Arch. Biochem. Biophys. 329: 113-122, 1996. PubMed: 8619627

38890: Huuskonen SE, et al. A fish hepatoma cell line (PLHC-1) as a tool to study cytotoxicity and CYP1A induction properties of cellulose and wood chip extracts. Chemosphere 36: 2921-2932, 1998. PubMed: 9734273

38891: Schultz ME, Schultz RJ. Transplantable chemically-induced liver tumors in the viviparous fish Poeciliopsis. Exp. Mol. Pathol. 42: 320-330, 1985. PubMed: 2987024

38892: Tocher DR, et al. Occurrence of 22:3n-9 and 22:4n-9 in the lipids of the topminnow (Poeciliopsis lucida) hepatic tumor cell line, PLHC-1. Lipids 30: 555-565, 1995. PubMed: 7651084

38897: Fent K. Ecotoxicology of organotin compounds. Crit. Rev. Toxicol. 26: 1-117, 1996. PubMed: 8833456

38898: Babich H, et al. PLHC-1細(xì)胞In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida). Ecotoxicol. Environ. Saf. 21: 327-336, 1991. PubMed: 1868789

38901: Ryan JA, Hightower LE. Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay. Environ. Tox. Chem. 13: 1231-1240, 1994.

38902: Bruschweiler BJ, et al. Inhibitory effects of heavy metals on cytochrome P4501A induction in permanent fish hepatoma cells. Arch. Environ. Contam. Toxicol. 31: 475-482, 1996. PubMed: 8975819

38904: Bruschweiler BJ, et al. Cytotoxicity in vitro of organotin compounds to fish hepatoma cells PLHC-1 (Poecilopsis lucida). Aquat. Toxicol. 32: 143-160, 1995.

38907: Hahn ME, et al. Cytochrome P4501A induction and inhibition by 3,3',4,4'-tetrachlorobiphenyl in an Ah receptor-containing fish hepatoma cell line (PLHC-1). Aquat. Toxicol. 26: 185-208, 1993.

38921: Bruschweiler BJ, et al. Inhibition of cytochrome P4501A by organotins in fish hepatoma cells PCHC-1. Environ. Tox. Chem. 15: 728-735, 1996.

PLHC-1細(xì)胞38922: Bruschweiler BJ, et al. An ELISA asay for cytochrome P4501A in fish liver cells. Environ. Tox. Chem. 15: 592-596, 1996.

38923: Hahn ME, et al. Rapid assessment of induced cytochrome P4501A protein and catalytic activity in fish hepatoma cells grown in multiwell plates: response to TCDD, TCDF, and two planar PCBS. Environ. Tox. Chem. 15: 582-591, 1996.