CTX TNA2細(xì)胞

生長狀態(tài)： 貼壁生長

相關(guān)**： 正常

ATCC Number： CRL-2006?

細(xì)胞形態(tài)： 成纖維樣

物種來源： 大鼠

是否是腫瘤細(xì)胞： 0

細(xì)胞類型： 其他細(xì)胞類型

器官來源： 大腦

數(shù)量： 大量

年限： neonate

組織來源： cortex

CTX TNA2細(xì)胞品系： Sprague-Dawley

運(yùn)輸方式： 凍存運(yùn)輸

Designations: CTX TNA2

Depositors: CF Deschepper

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: fibroblast

Source: Organ: brain

Strain: Sprague-Dawley

Tissue: cortex

Disease: normal

Cell Type: astrocyte, type 1 phenotype;

Cellular Products: CTX TNA2細(xì)胞alpha 2 macroglobulin; transferrin

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Tumorigenic: No

Age: neonate

Comments: The CTX TNA2 cell line was established from primary cultures of type 1 astrocytes from brain frontal cortex tissue of 1 day old rats.

The cultures were transfected 3 days after initial plating with a DNA construct containing the oncogenic early region of SV40 under the transcriptional control of the human GFAP promoter (pGFA-SV-Tt).

And pPGK-neo which contains the murine phosphoglycerate kinase gene promoter.

The transfectants were selected with G418 and cloned.

The cells retain characteristics consistent with the phenotype of type 1 astrocytes.

About 20% of the cells have glial fibrillary acidic protein (GFAP) immunoreactivity.

CTX TNA2細(xì)胞The cells have a high affinity uptake mechanism for gamma aminobutyric acid (GABA) that is inhibitable by beta alanine.

The cells produce alpha 2 macroglobulin in amounts similar to those found in primary astrocytes but produce transferrin in much lesser amounts.

This line does not produce proenkephalin A, does not express the O4 or A2B5 epitopes characteristic of type 2 astrocytes, and does not express galactocerebroside.

SV40 T-antigen was found in the nuclei of over 95% of the cells examined by immunostaining.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Remove spent medium, add fresh 0.25% trypsin, 0.53mM EDTA solution, rinse and remove.

Add fresh trypsin solution, (1 to 2 ml) and allow the cells to sit at room temperature (or at 37C) until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

NOTE: CTX TNA2細(xì)胞The cells will come off in sheets and are difficult to dissociate.

Related Products: Trypsin EDTA Solution: ATCC 30-2101

References: 23333: Radany EH, et al. Directed establishment of rat brain cell lines with the phenotypic characteristics of type 1 astrocytes. Proc. Natl. Acad. Sci. USA 89: 6467-6471, 1992. PubMed: 1378628