266-6細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

器官來源： 胰腺

ATCC Number： CRL-2151?

相關(guān)**： 其他**

年限： *****

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: 266-6

Depositors: GH Swift

266-6細(xì)胞Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: pancreas

Disease: pancreatic acinar cell tumor

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1985

Receptors: acetylcholine, muscarinic

Age: *****

Comments: 266-6 is an acinar pancreatic cell line derived in 1985 by Robert E. Hammer from a young ***** mouse.

The tumor was induced with an elastase I/SV-40 T antigen fusion gene.

These cells retain a partially differentiated phenotype, and express detectable levels of a number of digestive enzyme mRNAs.

266-6細(xì)胞The cells respond to carbachol and cholecystokinin but not to substance P, secretin, or vasoactive intestinal peptide (VIP).

They bear an elastase I/neomycin transgene.

The 266-6 cell line is useful for transfection studies.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, and let the culture sit 37C for 3 minutes or until the cells detach. Add fresh medium, centrifuge at 1000 rpm for 8 minutes, resuspend the pellet in fresh medium and dispense into new gelatin coated flasks .Culture vessels must be coated prior to use with 0.1% pig skin gelatin in distilled water for 15 to 60 minutes. Suction off excess gelatin prior to use. The cells may be grown on untreated flasks, but growth will be clumpy. Growth on gelatin coated plates leads to better spreading of the cells.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium266-6細(xì)胞 (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22373: Kruse F, et al. The cell-specific elastase I enhancer comprises two domains [published erratum appears in Mol. Cell. Biol. 8: 1862, 1988]. Mol. Cell. Biol. 8: 893-902, 1988. PubMed: 3352608

22391: Swift GH, et al. Differential requirements for cell-specific elastase I enhancer domains in transfected cells and transgenic mice. Genes Dev. 3: 687-696, 1989. PubMed: 2744460

22621: Ornitz DM, et al. Elastase I promoter directs expression of human growth hormone and SV40 T antigen genes to pancreatic acinar cells in transgenic mice. Cold Spring Harbor Symp. Quant. Biol. 50: 399-409, 1985. PubMed: 3006998

23245: Rose SD, et al. A single element of the elastase I enhancer is sufficient to direct transcription selectively to the pancreas and gut. Mol. Cell. Biol. 14: 2048-2057, 1994. PubMed: 8114736

266-6細(xì)胞23532: Swift GH, et al. An element of the elastase I enhancer is an overlapping bipartite binding site activated by a heteromeric factor. J. Biol. Chem. 269: 12809-12815, 1994. PubMed: 8175694