Sp2/0-Ag14細(xì)胞

器官來源： 脾

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1581?

細(xì)胞形態(tài)： **樣

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

品系： BALB/c

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: Sp2/0-Ag14

Depositors: G Kohler

Sp2/0-Ag14細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Strain: BALB/c

Cell Type: hybridoma: B lymphocyte;

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Applications: fusion partner for production of somatic cell hybrids

Antigen Expression: H-2d

Sp2/0-Ag14細(xì)胞Comments: The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. [1077]

The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 mcg/ml and are HAT sensitive. [1077]

Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas. [906]

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain cell density between 5 X 10 (4) and 5 X 10 (5) viable cells/ml. Some cells can attach and be transferred by shaking them loose.

Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium Sp2/0-Ag14細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

derivative:ATCC CRL-1581.1

References: 906: Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293

1077: Shulman M, et al. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270, 1978. PubMed: 714156

32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

33167: Chen H, et al. Sp2/0-Ag14細(xì)胞Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022