TALL-104細(xì)胞
數(shù)量： 大量
年限： 2 years
細(xì)胞類型： T**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 人
生長狀態(tài)： 懸浮生長
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-11386?
相關(guān)**： 白血病
細(xì)胞形態(tài)： **樣
Designations: TALL-104
Depositors: Wistar Institute
Biosafety Level: 1
TALL-104細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast;
Cellular Products: interferon gamma (IFN gamma); tumor necrosis factor alpha (TNF alpha); granulocyte monocyte colony stimulating factor (GM-CSF)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: T cell antigen receptor alpha/beta (TCR), expressed
interleukin 2 (IL-2), expressed
interleukin 2 (IL-2); T cell antigen receptor alpha/beta (TCR)
Antigen Expression: CD2 +; CD3 +; CD7 +; CD8 +; CD56 +; CD4 -; CD16 -
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 12,13
TALL-104細(xì)胞D7S820: 7,13
THO1: 7,9
TPOX: 8,9
vWA: 14,18
Cytogenetic Analysis: 46, XY; t(11;14)(p13;q11)
Age: 2 years
Gender: male
Comments: The TALL-104 line was established from the peripheral blood of a child in relapse with T-ALL.
The cells are highly cytotoxic and are capable of tumor destruction in vivo and in vitro.
IL-2 is required from optimal growth and long term cultivation, but the cells can be grown for short periods without IL-2 (growth will be slower).
The cells are positive for the alpha/beta TCR and negative for gamma/delta TCR.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. ATCC 30-2005. To make the complete growth medium, add the following components to the base medium: 50 to 100 units/ml recombinant human IL-2; 2.5 microgram/ml human albumin; 0.5 microgram/ml D-mannitol; fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; TALL-104細(xì)胞carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Successful growth of this cell line is very dependent upon the quality of IL-2 used in the growth medium. ATCC recommends using the highest quality IL-2 available.
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at least 8 X 10(5) cells/ml.
Note: IL-2 rapidly loses its potency in medium, it is important that fresh IL-2 be used.
Interval: Maintain between 4 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add medium as cell density increases
Preservation: Freeze medium: Complete growth medium supplemented with 50% fetal bovine serum, and 10% DMSO. Freeze at a cell density of at least 15 X 10(6) cells/ml
Storage temperature: liquid nitrogen vapor phase
References: 22047: TALL-104細(xì)胞Santoli D, et al. Cytotoxic T-ALL cell lines and uses therefor. US Patent 5,272,082 dated Dec 21 1993
23356: O'Connor R, et al. Growth factor requirements of childhood acute T-lymphoblastic leukemia: correlation between presence of chromosomal abnormalities and ability to grow permanently in vitro. Blood 77: 1534-1545, 1991. PubMed: 1706955
23357: Cesano A, et al. Homing and progression patterns of childhood acute lymphoblastic leukemias in severe combined immunodeficiency mice. Blood 77: 2463-2474, 1991. PubMed: 2039829
