WSS-1細(xì)胞

ATCC Number： CRL-2029?

生長狀態(tài)： 貼壁生長

細(xì)胞形態(tài)： 上皮樣

運輸方式： 凍存運輸

細(xì)胞類型： 其他細(xì)胞類型

年限： fetus

是否是腫瘤細(xì)胞： 0

物種來源： 人

器官來源： 腎臟

數(shù)量： 大量

Designations: WSS-1 [WS-1]

WSS-1細(xì)胞Depositors: G Wong

Biosafety Level: 2 [CELLS CONTAIN ADENOVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: kidney

Cell Type: transformed with adenovirus 5 DNA

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Applications: produces protein gamma-aminobutyric acid (GABA) A receptor, alpha 1 [41464]

Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits). [41464]

Handle as potentially biohazardous material under at least Biosafety Level 2 containment. [41464]

Receptors: vitronectin; gamma-aminobutyric acid - A receptor (GABA-A, Type 1 BzR)

DNA Profile (STR): WSS-1細(xì)胞Amelogenin: X

CSF1PO: 12

D13S317: 12,14

D16S539: 9,13

D5S818: 8

D7S820: 11,12

THO1: 7,9.3

TPOX: 11

vWA: 16,19

Age: fetus

Comments: This line was derived from the human embryonic kidney line, 293 (see ATCC CRL-1573). 293 cells were transfected with an expression plasmid containing cDNA encoding the rat GABA-A receptor alpha 1, beta 2 and gamma 2 subunits. Genomic integration of the transfected alpha and gamma subunit genes was confirmed; however, the beta subunit sequences were not detected in Northern and Southern blots.

Propagation: WSS-1細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: parental cell line:ATCC CRL-1573

References: 41464: Wong G, et al. WSS-1細(xì)胞Stable expression of type I gamma-aminobutyric acidA/benzodiazepine receptors in a transfected cell line. Mol. Pharmacol. 42: 996-1003, 1992. PubMed: 1336119