72A1細胞
ATCC Number： HB-168?
生長狀態(tài)： 懸浮生長
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
數量： 大量
細胞形態(tài)： **樣
**類型： mouse IgG1
Designations: 72A1
Depositors: GJ Hoffman
Isotype: mouse IgG1
72A1細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Epstein-Barr virus (EBV)
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Comments: Animals were immunized with disrupted fixed Epstein-Barr virus particles.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the 350000/220000 dalton viral envelope glycoprotein.
Tested and found negative for ectromelia virus72A1細胞 (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml.
72A1細胞Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1172: Hoffman GJ, et al. Monoclonal antibody against a 250,000-dalton glycoprotein of Epstein-Barr virus identifies a membrane antigen and a neutralizing antigen. Proc. Natl. Acad. Sci. USA 77: 2979-2983, 1980. 72A1細胞PubMed: 6248876
