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P3X63Ag8.653細胞

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產(chǎn)品名稱: P3X63Ag8.653細胞
產(chǎn)品型號: P3X63Ag8.653
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

P3X63Ag8.653細胞應(yīng)如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。P3X63Ag8.653細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


P3X63Ag8.653細胞  的詳細介紹

P3X63Ag8.653細胞

品系: BALB/c

細胞類型: B**細胞

是否是腫瘤細胞: 0

物種來源: 小鼠

生長狀態(tài): 懸浮生長

ATCC Number: CRL-1580?

相關(guān)**: 多發(fā)性骨髓瘤

運輸方式: 凍存運輸

數(shù)量: 大量

細胞形態(tài): **樣

Designations: P3X63Ag8.653

Depositors: JF Kearney

P3X63Ag8.653細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast


Source: Disease: plasmacytoma; myeloma

Strain: BALB/c

Cell Type: B lymphoblast;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Antigen Expression: H-2d

Comments: P3X63Ag8.653細胞The cells are resistant to 8-azaguanine and are HAT sensitive.

They can be used as fusion partners for producing hybridomas.

The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 10(5) viable cells/ml and subculture before the cell density reaches 2 X 10(6) cells/ml.

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium P3X63Ag8.653細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1075: Kearney JF, et al. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123: 1548-1550, 1979. PubMed: 113458

23366: Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

32914: Oppermann M, et al. Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996. P3X63Ag8.653細胞PubMed: 8755530

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