TE3 [TE3A]細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG2

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-209?

TE3 [TE3A]細(xì)胞數(shù)量： 大量

Designations: TE3 [TE3A]

Depositors: BF Haynes

Isotype: IgG2

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human thymus (cortical thymic epithelium)

Permits/Forms: TE3 [TE3A]細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with human thymic stroma.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with an intracellular antigen expressed in cortical, but not medullary, thymic epithelium.

All TE3+ cells in the thymus were found to express HLA and Ia antigens, and contain keratin.

Propagation: ATCC complete growth medium: TE3 [TE3A]細(xì)胞Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1639: McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. TE3 [TE3A]細(xì)胞J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074