NFS-25 C-3細胞
生長狀態(tài)： 懸浮生長
細胞類型： 其他細胞類型
ATCC Number： CRL-1695?
相關(guān)**： **瘤
是否是腫瘤細胞： 0
物種來源： 小鼠
品系： NFS.C58v-1
數(shù)量： 大量
細胞形態(tài)： **樣
運輸方式： 凍存運輸
NFS-25 C-3細胞Designations: NFS-25 C-3
Depositors: WJ Davidson, HC Morse
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: NFS.C58v-1
Cell Type: pre-B lymphoblast;
Permits/Forms: NFS-25 C-3細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: CD5 +; CD32 +; CD45R +
Comments: The NFS-25 C-3 cell line was derived from a spontaneous lymphoma.
The cells bear markers consistent with identification as large pre-B lymphoblasts [(Ly 17, CD32), (Ly 1, CD5), (Lyb 2, Ly 5(B220), CD45R)].
They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 10 mM HEPES, 3 mM glutamine, 1 mM oxaloacetic acid, 0.2 units/ml insulin, 0.5 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, 2 mcg/ml transferrin, 80%; NCTC medium 109, 10%; fetal bovine serum, 10%
NFS-25 C-3細胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: 82.5% complete growth medium; 10% FBS; 7.5% DMSO.
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22836: Davidson WF, et al. NFS-25 C-3細胞A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201
