CMT-93細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
品系： C57BL/icrf
ATCC Number： CCL-223?
相關(guān)**： 其他**
數(shù)量： 大量
器官來(lái)源： 直腸
細(xì)胞形態(tài)： 上皮樣
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
年限： 19 months
運(yùn)輸方式： 凍存運(yùn)輸
Designations: CMT-93
CMT-93細(xì)胞Depositors: LM Franks
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Strain: C57BL/icrf
Organ: rectum
Disease: polyploid carcinoma
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Tumorigenic: Yes
Antigen Expression: H-2b
GenoType: a(t)
CMT-93細(xì)胞Age: 19 months
Gender: male
Comments: Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. CMT-93細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22753: Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum.CMT-93細(xì)胞 J. Pathol. 124: 35-38, 1978. PubMed: 722371
