RKO細胞
運輸方式： 凍存運輸
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-2577?
相關(guān)**： 腫瘤
細胞形態(tài)： 上皮樣
數(shù)量： 大量
器官來源： 結(jié)腸
是否是腫瘤細胞： 0
物種來源： 人
Designations: RKO
Depositors: MC Hollander, AJ Fornace
RKO細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: epithelial
Source: Organ: colon
Disease: carcinoma
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Receptors: urokinase receptor (u-PAR) [51462]
Tumorigenic: Yes
Oncogene: p53 + (wild type)
DNA Profile (STR): Amelogenin: X
CSF1PO: 8, 10
D13S317: 8, 11
D16S539: 12, 13
D5S818: 11, 13
D7S820: 8, 10
RKO細胞THO1: 6, 10
TPOX:11
vWA: 15, 16, 17
Comments: RKO is a poorly differentiated colon carcinoma cell line developed by Michael Brattain.
RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor (h-TRbeta1). The level of p53 protein is higher in RKO (ATCC CRL-2577) cells than in RKO-E6 (ATCC CRL-2578) cells. [53331]
The RKO cell line is the parental cell line (isogenic) of RKO-E6 (ATCC CRL-2578) and RKO-A545-1 (ATCC CRL-2579). It can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. RKO細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
derivative:ATCC CRL-2578
derivative:ATCC CRL-2579
References: 51462: Boyd D, et al. Determination of the levels of urokinase and its receptor in human colon carcinoma cell lines. Cancer Res. 48: 3112-3116, 1988. PubMed: 2835152
51463: Smith ML, et al. Involvement of the p53 tumor suppressor in repair of u.v.-type DNA damage. Oncogene 10: 1053-1059, 1995. PubMed: 7700629
53329: Brattain MG, et al. Heterogeneity of human colon carcinoma. Cancer Metastasis Rev. 3: 177-191, 1984. PubMed: 6437669
53331: Bhat MK, et al. RKO細胞Tumor suppressor p53 is a negative regulator in thyroid hormone receptor signaling pathways. J. Biol. Chem. 272: 28989-28993, 1997. PubMed: 9360971
