CRFK細(xì)胞

ATCC Number： CCL-94?

相關(guān)**： 正常

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 貓

年限： 12 weeks

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 腎皮質(zhì)

CRFK細(xì)胞Designations: CRFK

Depositors: RA Van Deusen

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Felis catus

Morphology: epithelial

Source: Organ: kidney, cortex

Disease: normal

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CRFK細(xì)胞Applications: transfection host

Age: 12 weeks

Gender: female

Comments: The cells are free of bovine viral diarrhea (BVD) virus.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and allow culture to stand at room temperature (or at 37C) until cells detach . CRFK細(xì)胞Add fresh medium, aspirate and dispense into new flasks.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

References: 26266: Crandell RA, et al. Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. PubMed: 4130570

26267: Fabricant CG, Rich LJ. Microbial studies of feline urolithiasis. J. Am. Vet. Med. Assoc. 158: 976-980, 1971. PubMed: 4930310

26268: Lee KM, et al. Use of an established cat cell line for investigation and quantitation of feline oncornaviruses. J. Natl. Cancer Inst. 49: 55-60, 1972. PubMed: 4338781

CRFK細(xì)胞28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321