M-NFS-60細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： CRL-1838?

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

Designations: M-NFS-60

M-NFS-60細(xì)胞Depositors: JF Weaver, A Creasey

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: myelogenous leukemia

Cell Type: virus induced

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Applications: transfection host

Comments: M-NFS-60細(xì)胞The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus.

The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF).

The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

0.05 mM 2-mercaptoethanol

62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF)

fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: M-NFS-60細(xì)胞Add fresh medium at the time of subculture

Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 2.5 X 10 exp4 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22842: Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

23295: Weinstein Y, et al. M-NFS-60細(xì)胞Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527