BC3H1細(xì)胞
品系： C3H
組織來(lái)源： smooth muscle like
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
器官來(lái)源： 大腦
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
BC3H1細(xì)胞ATCC Number： CRL-1443?
相關(guān)**： 腫瘤
Designations: BC3H1
Depositors: W Carlisle
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology:
Source: Organ: brain
Strain: C3H
Tissue: smooth muscle like
BC3H1細(xì)胞Disease: tumor
Cell Type: methylnitrosourea induced
Cellular Products: adenylate phosphokinase; creatine phosphokinase
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Receptors: acetylcholine
Antigen Expression: H-2k
Comments: Recent data suggest that BC3H1 cells may more closely resemble cells in an arrested state of skeletal muscle differentiation than smooth muscle cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: BC3H1細(xì)胞A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22488: Schubert D, et al. Characterization of a unique muscle cell line. J. Cell Biol. 61: 398-413, 1974. PubMed: 4363958
23423: Taubman MB, et al. The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation. J. Cell Biol. 108: 1799-1806, 1989. PubMed: 2715180
26075: Patrick J, et al. BC3H1細(xì)胞Acetylcholine receptor metabolism in a nonfusing muscle cell line. J. Biol. Chem. 252: 2143-2153, 1977. PubMed: 845167
