FHs 74 Int細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
年限： 3 to 4 months gestation
器官來(lái)源： 小腸
數(shù)量： 大量
ATCC Number： CCL-241?
相關(guān)**： 正常
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
細(xì)胞形態(tài)： 上皮樣
Designations: FHs 74 Int
FHs 74 Int細(xì)胞Depositors: R Owens
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: small intestine
Disease: normal
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Receptors: epidermal growth factor (EGF), expressed
Tumorigenic: No
DNA Profile (STR): FHs 74 Int細(xì)胞Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Cytogenetic Analysis: normal female; diploid
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1
Me-2, 1-2
PGM1, 1
PGM3, 1
Age: 3 to 4 months gestation
Gender: FHs 74 Int細(xì)胞female
Comments: PAS, negative.
The cells are negative for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10%
(Do not filter the medium after addition of EGF)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: FHs 74 Int細(xì)胞A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
26058: Smith HS, et al. FHs 74 Int細(xì)胞Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205
