HFN 7.1細(xì)胞

器官來(lái)源： 脾

細(xì)胞形態(tài)： **樣

**類型： IgG1

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： CRL-1606?

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

Designations: HFN 7.1

Depositors: RC Schoen, KL Bentley, RJ Klebe

Isotype: IgG1

HFN 7.1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human fibronectin

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Antigen Expression: HFN 7.1細(xì)胞H-2d

Comments: The antibody does not cross-react with other fibronectins tested (26 species including 15 primates).

Mice were immunized with purified human fibronectin.

Spleen cells were fused with P3X63Ag8 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Formerly ATCC HB-39.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. HFN 7.1細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml.

References: 57999: Schoen RC, et al. HFN 7.1細(xì)胞Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125