11F11細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

ATCC Number： CRL-1908?

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG2a; kappa light chain

Designations: 11F11

Depositors: AD O'Brien

Isotype: IgG2a; kappa light chain

11F11細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II)

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Comments: 11F11細(xì)胞Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with the A subunit of SLT-II and does not react with B subunit.

The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains.

It may also be used to differentiate SLT-II from SLT-I.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: 11F11細(xì)胞Medium Renewal: Every 2 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.

References: 1784: Perera LP, et al. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J. Clin. 11F11細(xì)胞Microbiol. 26: 2127-2131, 1988. PubMed: 3053764