BW5147.G.1.4細(xì)胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

細(xì)胞類型： T**細(xì)胞

品系： AKR/J

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： TIB-48?

相關(guān)**： **瘤

器官來源： 胸腺

Designations: BW5147.G.1.4

BW5147.G.1.4細(xì)胞Depositors: R Hyman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: thymus

Strain: AKR/J

Disease: lymphoma

Cell Type: T lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: This line was derived from BW5147.3 (ATCC TIB-47).

BW5147.G.1.4細(xì)胞The cells are resistant to 0.1 mM 6-thioguanine.

Unlike the parental line, these cells are sensitive to HAT.

Tested and found negative for ectromelia virus (mousepox).

Antigen Expression: H-2k

Comments: This line was derived from BW5147.3 (ATCC TIB-47).

The cells are resistant to 0.1 mM 6-thioguanine.

Unlike the parental line, these cells are sensitive to HAT.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. BW5147.G.1.4細(xì)胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

22348: Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329

22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

26346: Goldsby RA, et al. Hybrid cell lines with T-cell characteristics. Nature 267: 707-708, 1977. PubMed: 301614

26347: Hammerling GJ. BW5147.G.1.4細(xì)胞T lymphocyte tissue culture lines produced by cell hybridization. Eur. J. Immunol. 7: 743-746, 1977. PubMed: 304002