MYC 1-9E10.2 [9E10]細(xì)胞
ATCC Number： CRL-1729?
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgG1
Designations: MYC 1-9E10.2 [9E10]
MYC 1-9E10.2 [9E10]細(xì)胞Depositors: J Bishop, GI Evan
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human myc (c-myc) protein
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. MYC 1-9E10.2 [9E10]細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.
Applications: Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Tested and found negative for ectromelia virus (mousepox).
Comments: Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody precipitates the p62 c-myc protein, but does not react with mouse or chicken c-myc.
Tested and found negative for ectromelia virus (mousepox).
Propagation: MYC 1-9E10.2 [9E10]細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1189: Evan GI, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616, 1985. PubMed: 3915782
29119: Van Ewijk WV, et al. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments. Proc. Natl. Acad. Sci. USA 94: 3903-3908, 1997. PubMed: 9108077
33026: Cheng M, et al. MYC 1-9E10.2 [9E10]細(xì)胞ERK3 is a constitutively nuclear protein kinase. J. Biol. Chem. 271: 8951-8958, 1996. PubMed: 8621539
