CVC.1細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： TIB-135?
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgG1
Designations: CVC.1
Depositors: FM Brodsky, P Parham
CVC.1細(xì)胞Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against bovine brain clathrin (light chain, 36000 dalton)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with bovine brain clathrin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
CVC.1細(xì)胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 966: Kirchhausen T, et al. CVC.1細(xì)胞Location and distribution of the light chains in clathrin trimers. Proc. Natl. Acad. Sci. USA 80: 2481-2485, 1983. PubMed: 6189124
974: Brodsky FM, Parham P. Polymorphism in clathrin light chains from different tissues. J. Mol. Biol. 167: 197-204, 1983. PubMed: 6864801
