tsc2 ang1細(xì)胞
ATCC Number： CRL-2620?
數(shù)量： 大量
相關(guān)**： 其他**
生長狀態(tài)： 貼壁生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
Designations: tsc2 ang1
Depositors: JL Arbiser
Biosafety Level: 1
tsc2 ang1細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology:
Source: Disease: cutaneous sarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: tsc2 ang1細(xì)胞Tuberous sclerosis (TSC) is an autosomal dominant genetic disorder caused by mutations in either TSC1 or TSC2. Mice heterozygous for Tsc2 were developed as a murine model of Tsc2 disease using a gene targeting approach. [53493]
The tsc2 ang1 cell line was derived in 1998 from a cutaneous sarcoma taken from a Tsc2+/- mouse heterozygous for tuberin.
The cell line is a useful model of TSC. It is the only genetically defined tuberous sclerosis cell line available that forms tumors. It can be used for drug testing and for angiogenesis studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended
Medium Renewal: tsc2 ang1細(xì)胞Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 53493: Onda H, et al. Tsc2(+/-) mice develop tumors in multiple sites that express gelsolin and are influenced by genetic background. J. Clin. Invest. 104: 687-695, 1999.tsc2 ang1細(xì)胞 PubMed: 10491404
