Pl 1 Ut (NBL-9)細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

年限： *****

細(xì)胞形態(tài)： 成纖維樣

ATCC Number： CCL-74?

器官來源： **

是否是腫瘤細(xì)胞： 0

物種來源： 其他

生長狀態(tài)： 貼壁生長

Designations: Pl 1 Ut (NBL-9)

Depositors: AJ Kniazeff, WA Nelson-Rees, NB Darby, R Owens

Pl 1 Ut (NBL-9)細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Procyon lotor

Morphology: fibroblast

Source: Organ: uterus

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: poliovirus 2; coxsackievirus A9, B5; vaccinia; adenovirus 2, 5

Age: *****

Gender: female

Comments: Pl 1 Ut (NBL-9)細(xì)胞This cell line was developed for studies of feline and canine viruses since the raccoon is one of the few known animals which is susceptible to both of these groups of viruses.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Twice per week

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: Pl 1 Ut (NBL-9)細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

References: 28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. Pl 1 Ut (NBL-9)細(xì)胞PubMed: 8985321