SW 626細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

器官來(lái)源： 卵巢

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

年限： grade III

細(xì)胞形態(tài)： 上皮樣

ATCC Number： HTB-78?

相關(guān)**： 腺癌

Designations: SW 626 [SW-626, SW626]

Depositors: A Leibovitz

SW 626細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: ovary

Tumor Stage: grade III

Disease: adenocarcinoma

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Isolation: Isolation date: January, 1974

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11

SW 626細(xì)胞D16S539: 11

D5S818: 12

D7S820: 10,12

THO1: 9.3

TPOX: 8

vWA: 17,18

Cytogenetic Analysis: Hypertetraploid; modal number = 104. The rate of higher ploidies was 23%. The markers der(2)t(2;5)(q35;q31); del(8)(q13q22); del(12)(q13); t(q9q13) and two others were common to most cells. Generally there were two copies of der(2) and three copies of del(8) per cell. The t(3;11)(p21;q25) and i(15q) markers were seen in some cells. Many cells had 8 copies of N3, N7, N9, N19 and N20, but only two copies of N2. Normal 8 was absent. There were four copies of X, and Y was not found.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 1

PGM3, 1

Age: 46 years

Gender: female

Ethnicity: Caucasian

Comments: SW 626細(xì)胞Although originally thought to be of ovarian origin, a recent report has suggested that the SW 686 cell line might have been derived from an ovarian metastasis of a primary adenocarcinoma of the colon.

The SW 626 cell line was initiated by A. Leibovitz in January 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen from a cystadenocarcinoma of the ovary in a 46 year old female Caucasian.

The histopathology of the specimen was determined to be grade III adenocarcinoma.

A frozen ampule at passage 10 was received at the ATCC in January, 1982.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: SW 626細(xì)胞A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

32824: Strobel T, et al. BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway. Proc. Natl. Acad. Sci. USA 93: 14094-14099, 1996. PubMed: 8943066

40013: Furlong MT, et al. Evidence for the colonic origin of ovarian cancer cell line SW626. J. Natl. Cancer Inst. 91: 1327-1328, 1999. PubMed: 10433623