HS-5細胞
ATCC Number： CRL-11882?
運輸方式： 凍存運輸
細胞形態(tài)： 成纖維樣
數(shù)量： 大量
年限： 30 years
器官來源： 骨髓
生長狀態(tài)： 貼壁生長
組織來源： stroma
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 人
Designations: HS-5
Depositors: Fred Hutchinson Cancer Res. Cntr.
HS-5細胞Biosafety Level: 2 [Cells contain human papilloma viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: fibroblast
Source: Organ: bone marrow
Tissue: stroma
Cell Type: HPV-16 E6/E7 transformed
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Applications: HS-23 and HS-27A secrete low levels of growth factors and do not support proliferation of isolated progenitor cells in cocultures.
The cell line can also be used as a feeder layer in ex vivo bone marrow cultures or in colony forming assays.
Age: 30 years
Gender: male
HS-5細胞Ethnicity: Caucasian
Comments: Cellular Products: : granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), macrophage-CSF (M-CSF), Kit ligand (KL), macrophage-inhibitory protein-1 alpha, interleukin-1 alpha (IL-1alpha), IL-1beta, IL-1RA, IL-6, IL-8, IL-11, and leukemia inhibitory factor (LIF)
Long term bone marrow cells were transformed with the amphotropic retrovirus vector LXSN16E6E7 in the presence of polybrene.
Twenty-seven immortalized clones designed HS-1 to HS-27 were isolated.
One of these cell lines HS-27A (CRL-2496) has been deposited in the ATCC 's general collection. One cell line (HS-5) has been deposited in the Patent Depository (CRL-11882).
HS-5 supports proliferation of hematopoietic progenitor cells when cocultured in serum-deprived media with no exogenous factors.
HS-23 and HS-27A secrete low levels of growth factors and do not support proliferation of isolated progenitor cells in cocultures.
The cell line can also be used as a feeder layer in ex vivo bone marrow cultures or in colony forming assays.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
HS-5細胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:9 is recommended
Medium Renewal: Everh 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 40180: HS-5細胞Roecklein BA, Torok-Storb B. Functionally distinct human marrow stromal cell lines immortalized by transduction with the human papilloma virus E6/E7 genes. Blood 85: 997-1005, 1995. PubMed: 7849321
44213: Torok-Storb B, et al. Human marrow stromal cell lines which sustain hematopoieses. US Patent 5,879,940 dated Mar 9 1999
