CCD-19Lu細(xì)胞 ATCC細(xì)胞

細(xì)胞類(lèi)型： 成纖維細(xì)胞

細(xì)胞形態(tài)： 成纖維樣

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

數(shù)量： 大量

ATCC Number： CCL-210?

相關(guān)**： 正常

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 肺

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： 20 years

Designations: CCD-19Lu

Depositors: S Dilworth

CCD-19Lu細(xì)胞 ATCC細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. CCD-19Lu細(xì)胞

Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 11,12

D16S539: 11

D5S818: 12

D7S820: 9,11

THO1: 9.3

TPOX: 8

vWA: 17,18

Cytogenetic Analysis: normal female; diploid; stable

Age: 20 years

Gender: female

Ethnicity: Caucasian

Comments: CCD-19Lu細(xì)胞The line was derived from normal tissue from patient who died of trauma to the head.

The cells are capable of at least 8 or 9 population doublings beyond the current freeze.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for two minutes at room temperature. Remove trypsin and let the culture stand at 37C for five minutes. Add fresh medium, aspirate and dispense into new flasks. CCD-19Lu細(xì)胞Subculture every 6 to 8 days.