JAWSII細(xì)胞

生長(zhǎng)狀態(tài)： 混合型生長(zhǎng)

品系： C57BL/6

ATCC Number： CRL-11904?

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

細(xì)胞形態(tài)： 單核細(xì)胞/巨噬細(xì)胞

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 骨髓

Designations: JAWSII

JAWSII細(xì)胞Depositors: ZymoGenetics, Inc.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Mus musculus

Morphology: monocyte

Source: Organ: bone marrow

Strain: C57BL/6

Cell Type: immature dendritic cell; monocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. JAWSII細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Propagation: ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM L-glutamine, 1 mM sodium pyruvate and 5 ng/ml murine GM-CSF, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by transferring floating cells to a centrifuge tube.

Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.

Pool cells and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.

Note: This cell line grows very slowly.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Once per week

Preservation: JAWSII細(xì)胞Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

References: 38868: MacKay VL, Moore EE. Immortalized dendritic cells. US Patent 5,648,219 dated Jul 15 1997

47440: Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998