DDT1 MF-2細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 其他
組織來(lái)源： smooth muscle
細(xì)胞形態(tài)： 上皮樣
器官來(lái)源： 其他
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
數(shù)量： 大量
ATCC Number： CRL-1701?
相關(guān)**： 其他**
Designations: DDT1 MF-2
Depositors: JS Norris
DDT1 MF-2細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mesocricetus auratus
Morphology: epithelial
Source: Organ: ductus deferens
Tissue: smooth muscle
Disease: leiomyosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Receptors: androgen receptor, positive; glucocorticoid; alpha 1 adrenergic; beta 2 adrenergic
Gender: male
Comments: DDT1 MF-2細(xì)胞Growth of DDT1 MF-2 cells is stimulated by androgens and inhibited by glucocorticoids.
Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene.
Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week
To dislodge adherent cells, remove all but 5 to 10 ml of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. DDT1 MF-2細(xì)胞Subcultures may also be prepared using standard trypsinization procedures.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 2153: Syms AJ, et al. Proliferation of a highly androgen-sensitive ductus deferens cell line (DDT MF-2) is regulated by glucocorticoids and modulated by growth collagen. In Vitro 19: 929-936, 1983. PubMed: 6662552
22891: Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513
26146: Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. DDT1 MF-2細(xì)胞PubMed: 6743325
