PFHR 9細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： CRL-2423?

相關(guān)**： 其他**

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

年限： embryo

Designations: PFHR 9 [PFHR-9]

Depositors: A Chung

Biosafety Level: 1

PFHR 9細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus deposited as mouse

Morphology: epithelial

Source: Disease: embryonal carcinoma

Cellular Products: laminin-1; entactin-1; perlecan and collagen IV

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Age: embryo

Comments: PFHR 9細(xì)胞PFHR 9 (PFHR-9)is a differentiated cell line isolated as a HAT-resistant colony of the undifferentiated mouse embryonal carcinoma PCC4F in 1974.

While PCC4F cells grow in suspension, the PRHR cell line is adherent. The cells are epithelial-like and have numerous vacuoles. PFHR 9 cells have a reported doubling time of 12 hours.

The PFHR 9 cells were not tumorigenic when injected subcutaneously into 129/Sv mice whereas the parental line did produce tumors.

The cells produce basement membrane proteins - laminin-1; entactin-1; perlecan and collagen IV.

The cell line is useful for the production of basement membrane proteins and as a model to study the assembly and secretion of these proteins.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution.PFHR 9細(xì)胞 Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Doubling Time: 12 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 38748: Chung AE, et al. Morphological and biochemical observations on cells derived from the in vitro differentiation of the embryonal carcinoma cell line PCC4-F. Cancer Res. 37: 2072-2081, 1977. PFHR 9細(xì)胞PubMed: 558822