LA-4細(xì)胞

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

品系： A/He

年限： 28 weeks

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 肺

ATCC Number： CCL-196?

相關(guān)**： 其他**

數(shù)量： 大量

細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

LA-4細(xì)胞Designations: LA-4

Depositors: GD Stoner

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: lung

Strain: A/He

Disease: adenoma

Cell Type: urethan induced

Permits/Forms: LA-4細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis: Unstable karyotype. Range = 38 to 256. Metacentric chromosomes are noted in about 12% of the cells.

Age: 28 weeks

Comments: Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: LA-4細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete culture medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 23023: Stoner GD, et al. Clonal isolation of epithelial cells from mouse lung adenoma. Cancer Res. 35: 2177-2185, 1975. PubMed: 167947

58031: Stoner GD, et al. LA-4細(xì)胞Epithelial cells cultured from mouse lung adenoma: biosynthesis of lecithin and comparison of phospholipids with the lung. Exp. Mol. Pathol. 29: 102-114, 1978. PubMed: 581072