NCI-H2405細(xì)胞

ATCC Number： CRL-5944?

相關(guān)**： 非小細(xì)胞肺癌

運輸方式： 凍存運輸

數(shù)量： 大量

器官來源： 肺

生長狀態(tài)： 混合型生長

年限： stage 4

是否是腫瘤細(xì)胞： 0

物種來源： 人

Designations: NCI-H2405 [H2405]

Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

NCI-H2405細(xì)胞Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage 4

Disease: adenocarcinoma; non-small cell lung cancer

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., NCI-H2405細(xì)胞Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

Age: 47 years

Gender: male

Ethnicity: Caucasian

Comments: The line was established in September 1990.

The patient was a smoker.

75 pack years.

Propagation: NCI-H2405細(xì)胞ATCC complete growth medium: ACL-4 medium (serum-free)

The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

0.02 mg/ml insulin

0.01 mg/ml transferrin

25 nM sodium selenite (final conc.)

50 nM Hydrocortisone (final conc.)

1 ng/ml Epidermal Growth Factor (do not filter)

0.01 mM ethanolamine (final conc.)

0.01 mM phosphorylethanolamine (final conc.)

100 pM triiodothyronine (final conc.)

0.5% (w/v) bovine serum albumin (final conc.)

0.5 mM sodium pyruvate (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5mM)

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Cultures can be maintained by the addition of fresh mediumor replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cells and as attached epithelial-like cells. Attached cells may be subcultured using 0.25% trypsin - 0.53mM EDTA.

Subcultivation Ratio:: 1:5 to 1:8 is recommended.

Preservation: Freeze medium: 85% Culture Media + 10% FBS+ 5% DMSO

Storage temperature: NCI-H2405細(xì)胞liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.