BJ細(xì)胞

器官來源： 皮膚

運(yùn)輸方式： 凍存運(yùn)輸

年限： newborn

細(xì)胞形態(tài)： 成纖維樣

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

ATCC Number： CRL-2522?

相關(guān)**： 正常

細(xì)胞類型： 成纖維細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 人

BJ細(xì)胞Designations: BJ

Depositors: JR Smith

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: fibroblast

Source: Organ: skin; foreskin

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 8,9

D16S539: 9,13

D5S818: 12

D7S820: 11,12

THO1: 7,8

TPOX: 10,11

BJ細(xì)胞vWA: 16,18

Cytogenetic Analysis: [49835]

Age: newborn

Gender: male

Comments: The line was established from skin taken from normal foreskin.

The BJ cell line has a long lifespan in comparison with other normal human fibroblast cell lines.

A frozen ampule at population doubling 2.3 was received at the ATCC in April, 2000.

These cells have a reported normal diploid karyotype at population doubling 61 but an abnormal karyotype at population doubling 82. [49835]

They are telomerase negative. [44175]

The cells may be used for stable transfection studies. [47354]

Cells from ATCC ampules have the capacity to proliferate to a maximum of 72 population doublings before the onset of senescence.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

BJ細(xì)胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:9 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 44175: Yi X, et al. Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels. Mol. Cell. Biol. 19(6): 3989-3997, 1999. BJ細(xì)胞PubMed: 10330139

47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332

48461: Huffman KE, et al. Telomere shortening is proportional to the size of the G-rich telomeric 3'-overhang. J. Biol. Chem. 275: 19719-19722, 2000. PubMed: 10787419

49835: Morales CP, et al. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nat. Genet. 21: 115-118, 1999. PubMed: 9916803