TE4細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： IgM
ATCC Number： HB-207?
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
Designations: TE4
Depositors: BF Haynes
TE4細(xì)胞Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human thymus (thymic endocrine epithelium)
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Comments: TE4細(xì)胞Animals were immunized with human thymic stroma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with an intracellular antigen expressed in the endocrine thymic epithelium.
It defines the mesodermal portion of the thymic microenvironment that may participate in conferring MHC restriction to maturing T lymphocytes.
TE4+ cells strongly express class I and class II histocompatibility antigens, and are positive for thymosin alpha-1 and keratin.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: TE4細(xì)胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1638: Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. TE4細(xì)胞PubMed: 6200562
