P3/NSI/1-Ag4-1細(xì)胞

生長狀態(tài)： 懸浮生長

細(xì)胞形態(tài)： **樣

**類型： kappa light chain

品系： BALB/c

ATCC Number： TIB-18?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 多發(fā)性骨髓瘤

細(xì)胞類型： B**細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: P3/NSI/1-Ag4-1 [NS-1]

P3/NSI/1-Ag4-1細(xì)胞Depositors: G Kohler, C Milstein

Isotype: kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: plasmacytoma; myeloma

Strain: BALB/c

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin (not secreted)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. P3/NSI/1-Ag4-1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: H-2d

Comments: This is a non-secreting clone of P3X63Ag8 (ATCC TIB-9).

Kappa chains are synthesized but not secreted.

The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium.

The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: P3/NSI/1-Ag4-1細(xì)胞air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow the cell density to exceed 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liqid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC TIB-9

References: 956: Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938

1115: Kohler G, et al. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur. J. Immunol. 6: 292-295, 1976. PubMed: 825374

22154: Horibata K, Harris AW. P3/NSI/1-Ag4-1細(xì)胞Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

22347: Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377

23366: Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

26333: Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851

26344: Cowan NJ, et al. Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA. J. Mol. Biol. 90: 691-701, 1974. PubMed: 4449137

33092: Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. P3/NSI/1-Ag4-1細(xì)胞PubMed: 8662742