NBT-II細胞

運輸方式： 凍存運輸

數(shù)量： 大量

器官來源： 膀胱

品系： Wistar

ATCC Number： CRL-1655?

相關**： 腫瘤

生長狀態(tài)： 貼壁生長

是否是腫瘤細胞： 0

物種來源： 大鼠

細胞形態(tài)： 上皮樣

Designations: NBT-II [Nara Bladder Tumor No. 2]

Depositors: J Leighton, R Tchao

NBT-II細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: urinary bladder

Strain: Wistar

Disease: tumor

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Gender: male

Comments: NBT-II cells differentiate in vitro giving rise to tumor giant cells.

The differentiation is inhibited by vitamin A.

The line was derived from a tumor which was induced with N-butyl-N-(4-hydroxybutyl) nitrosamine.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. NBT-II細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22521: NBT-II細胞Toyoshima K, et al. Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(- 4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II. J. Natl. Cancer Inst. 47: 979-985, 1971. PubMed: 4941696

23029: Geisinger KR, et al. Appearance of giant cells as a morphological response of rat bladder carcinoma cell line (Nara Bladder Tumor No. 2) to a continuous thermal gradient in tissue culture. Cancer Res. 38: 1223-1230, 1978. PubMed: 639055

26131: Toyoshima K, Leighton J. Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture. Cancer Res. 35: 1873-1879, 1975. PubMed: 1093679

26132: Tchao R. Novel forms of epithelial cell motility on collagen and on glass surfaces. NBT-II細胞Cell Motil. 2: 333-341, 1982. PubMed: 7151140