PC-12細(xì)胞

生長狀態(tài)： 漂浮生長

器官來源： 腎上腺

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

運輸方式： 凍存運輸

數(shù)量： 大量

細(xì)胞形態(tài)： 其他

ATCC Number： CRL-1721?

相關(guān)**： 其他**

Designations: PC-12

Depositors: B Patterson

PC-12細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: floating clusters; few scattered lightly attached cells.

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: small irregularly shaped cells

Source: Organ: adrenal gland

Disease: pheochromocytoma

Cellular Products: catecholamines; dopamine; norepinephrine

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Applications: transfection host

Receptors: nerve growth factor (NGF), expressed

Tumorigenic: Yes

Cytogenetic Analysis: 40 chromosomes; 38 autosomes plus XY [1163]

Gender: PC-12細(xì)胞male

Comments: The PC-12 cell line was derived from a transplantable rat pheochromocytoma.

The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks.

The cells do not synthesize epinephrine.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

heat-inactivated horse serum to a final concentration of 10%

fetal bovine serum to a final concentration of 5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Transfer cell suspension to centrifuge tube. Centrifuge cells at 180 to 225 xg for 8-15 minutes at room temperature.

Remove and discard supernatant leaving cell pellet.

Resuspend the cell pellet in an appropriate volume of fresh medium (about one tenth of the original volume.

PC-12細(xì)胞Gently aspirate each 5 ml aliquot of cells 4 or 5 times with a new 20 ml syringe outfitted with a 22g (1? in.) needle to break up cell clusters.

Add appropriate aliquots of the cell suspension to new 75 cm2 flask with 10-15 ml fresh growth medium. Seed flask 5 x 10(5) to 1 x 10(6) viable cells/ml or use subcultivation ratio of 1:2 to 1:4.

Place culture vessels in incubator at 37?C Subculture when cell density reaches between 2-4 x 10(6) viable cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 48 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

Related cell line: ATCC CRL-1721.1, PC-1

References: 1162: Levi A, et al. PC-12細(xì)胞Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

1163: Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

22344: Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

33014: Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125

16173681: U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005