HO-13-4細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgM

生長狀態(tài)： 懸浮生長

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： TIB-99?

數(shù)量： 大量

Designations: HO-13-4

Depositors: A Marshak-Rothstein

Isotype: IgM

Biosafety Level: 1

HO-13-4細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Thy-1.2

Permits/Forms: HO-13-4細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with thymocytes from C3H mice.

Spleen cells were fused with P3X63Ag8 myeloma cells.

The antibody has only a slight cross-reaction with Thy-1.1.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: HO-13-4細(xì)胞fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: HO-13-4細(xì)胞Medium Renewal: Every 2 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

References: 893: Marshak-Rothstein A, et al. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J. Immunol. 122: 2491-2497, 1979. PubMed: 87465