HeLa [Chang Liver]細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

數(shù)量： 大量

器官來(lái)源： 其他

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CCL-13?

細(xì)胞形態(tài)： 上皮樣

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Designations: HeLa [Chang Liver]

Depositors: RS Chang

Biosafety Level: 2HeLa [Chang Liver]細(xì)胞 [Cells contain human papilloma virus (HPV-18) ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: HeLa contaminant

Cellular Products: keratin

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Applications: transfection host

Tumorigenic: Yes

DNA Profile (STR): HeLa [Chang Liver]細(xì)胞Amelogenin: X

CSF1PO: 10

D13S317: 12,13.3

D16S539: 9,10

D5S818: 12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18

Isoenzymes: G6PD, A

HeLa [Chang Liver]細(xì)胞HeLa Markers: Y

Comments: This line was originally thought to be derived from normal liver tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

The cells are positive for keratin by immunoperoxidase staining.

NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow flask to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Complete growth medium, 95%; DMSO, 5%

References: 22213: Chang RS. HeLa [Chang Liver]細(xì)胞Continuous subcultivation of epithelial-like cells from normal human tissues. Proc. Soc. Exp. Biol. Med. 87: 440-443, 1954. PubMed: 13237268

26122: Murphy WH, Landau BJ. Clonal variation and interaction of cells with viruses. Natl. Cancer Inst. Monogr. 7: 249-271, 1962. PubMed: 14477443

32352: Benn J, et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004