M17/4.4.11.9細胞

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 大鼠

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

**類型： rat IgG2a kappa

ATCC Number： TIB-217?

器官來源： 脾

運輸方式： 凍存運輸

Designations: M17/4.4.11.9 (new clone of M17/4.2)

M17/4.4.11.9細胞Depositors: TA Springer

Isotype: rat IgG2a kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Cell Type: hybridoma: M17/4.4.11.9細胞B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against the alpha subunit of LFA-1 (mouse cytotoxic T cell antigen, CD11a)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [993]

Comments: Animals were immunized with cytotoxic T cells from C57BL/6 mice immunized with P815 cells.

Spleen cells were fused with NS-1 myeloma cells.

LFA-1 is a cell surface antigen found on cytolytic T lymphocytes.

M17/4.4.11.9細胞Monoclonal antibodies against LFA-1 can block killing by inhibiting the adhesion between cytolytic T cells and target cells.

The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta.

The beta chain of LFA-1 (Leukocyte Function Associated antigen) is immunologically identical to the beta chain of the Mac-1 antigen.

The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC .

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

0.05 mM 2-mercaptoethanol

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml.

Interval: Maintain between 5 X 10(4) and 5 X 10(5) cells/ml.

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 993: Sanchez-Madrid F, et al. M17/4.4.11.9細胞Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and MAC-1. J. Exp. Med. 158: 586-602, 1983. PubMed: 6193226

58014: Sanchez-Madrid F, et al. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell. Immunol. 73: 1-11, 1982. PubMed: 6983917