GH4C1細(xì)胞

年限： 7 months

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 垂體

生長狀態(tài)： 漂浮生長

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

品系： Wistar-Furth

細(xì)胞形態(tài)： 上皮樣

ATCC Number： CCL-82.2?

數(shù)量： 大量

相關(guān)**： 腫瘤

Designations: GH4C1

GH4C1細(xì)胞Depositors: AH Tashjian

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: loosely adherent with floating clusters

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: pituitary

Strain: Wistar-Furth

Disease: tumor

Cellular Products: prolactin; growth hormone (somatotrophin)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis: modal number = 65; range = 60 to 68.

This is a hypertriploid rat cell line with the modal number of 65, occurring in 32% of cells. However, cells with 67 chromosomes also occurred at a high rate (22%). GH4C1細(xì)胞Cells with a higher ploidy count occurred at 0.6%. There were 25 to 30 marker chromosomes per cell including paired T(1;2-), single 4p+, chromosome 2 with the deleted q16-q24 (?), 11p+ and about 15 other constitutive markers. Only a single copy of the X chromosome was identified. Other characteristics included consistent single copies for chromosomes 1 and 3, double copies for chromosomes 5 and 8, and more than four copies for chromosome 7.

Age: 7 months

Gender: female

Comments: The GH4C1 cell line was developed in 1972 by A.H. Tashjian, Jr. from serially propagated GH3 (ATCC CCL-82.1) cells that suddenly produced little or no detectable levels of growth hormone.

GH4C1 cells differed from GH3 cells in that basal levels of growth hormone production were low (0.06 or less hormone/mg cell protein/24 hours.

Prolactin is also produced at a rate of 0.006 to 0.012 mg hormone/mg cell protein/24 hours.

Thyrotropin-releasing Hormone (TRH) stimulates prolactin synthesis and secretion.

Cloned cDNA from GH4C1 cells has been used to investigate the structure and regulation of the rat TRH receptor.

This line provides a system for studying the molecular mechanisms of action of hypothalamic peptides on growth hormone and prolactin release and synthesis.

The cells provide a system for studying the molecular mechanisms of receptor modulation and signal transduction in pituitary - derived cells.

GH4C1細(xì)胞They may be used for electrophysiological studies on plasma membrane calcium channels and also for studies of intracellular calcium homeostasis in secretory cells.

These cells have a plating efficiency of approximately 22% in the recommended culture medium.

Propagation: ATCC complete growth medium: Ham's F10 medium, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it.

Add 2.0 to 3.0 ml of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:20 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040

parental cell line:ATCC CCL-82.1

References: 881: Tashjian AH Jr., et al. GH4C1細(xì)胞Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281

21885: . Tissue Culture: methods and applications. New York: Academic Press; 1973.

22421: Takemoto H, et al. Adrenotropic activity of mammo-somatotropic tumors in rats and mice. I. Biologic aspects. Cancer Res. 22: 917-924, 1962. PubMed: 13984648

22573: Tashjian AH Jr.. Clonal strains of hormone-producing pituitary cells. Methods Enzymol. 58: 527-535, 1979. PubMed: 218079

23514: Zhao D, et al. Molecular cloning of a complementary deoxyribonucleic acid encoding the thyrotropin-releasing hormone receptor and regulation of its messenger ribonucleic acid in rat GH cells [published erratum appears in Endocrinology 132: 2658, 1993]. Endocrinology 130: 3529-3536, 1992. PubMed: 1317787

23543: Albert PR, Tashjian AH Jr.. Relationship of thyrotropin-releasing hormone-induced spike and plateau phases in cytosolic free Ca2+ concentrations to hormone secretion. Selective blockade using ionomycin and nifedipine. J. Biol. Chem. 259: 15350-15363, 1984. PubMed: 6439720